stress response induced by carbon nanoparticles in paracentrotus lividus
نویسندگان
چکیده
members of the 14-3-3 protein family are involved in many important cellular events, including stress response, survival and apoptosis. genes of the 14-3-3 family are conserved from plants to humans, and some members are responsive to uv radiation. despite the high rate of pollution generated by nano-pollutants, up to now is totally obscure their toxic effect on development. embryos treated with carbon nanoparticles, rna preparation, retro-transcription and quantitative real-time pcr. in response to carbon nano-particles exposure, the embryos collected 24 h later showed a 3,07-fold at 5x1012 p and a 1,58-fold at 2.5x1013 p and a 1,92-fold at 2.5x1014 p increase in pl14-3-3ε transcript levels compared with controls. the pl14-3-3ε mrna delocalization parallels the failure in archenteron elongation observed morphologically, as well as the lack of specific endoderm markers. here, we report the isolation of the complete cdna encoding the 14-3-3 epsilon isoform from paracentrotus lividus sea urchin embryos, referred to as pl14-3-3ε. pl14-3-3ε mrna levels were measured by rt-pcr during development and found to increase from the mesenchyme blastula to the prism stage. our results confirm the involvement of 14-3-3ε in the stress response elicited by carbon nano-particles.
منابع مشابه
Stress response induced by carbon nanoparticles in Paracentrotus lividus
Members of the 14-3-3 protein family are involved in many important cellular events, including stress response, survival and apoptosis. Genes of the 14-3-3 family are conserved from plants to humans, and some members are responsive to UV radiation. Despite the high rate of pollution generated by nano-pollutants, up to now is totally obscure their toxic effect on development. Embryos treated wit...
متن کاملStress response induced by carbon nanoparticles in Paracentrotus lividus
Members of the 14-3-3 protein family are involved in many important cellular events, including stress response, survival and apoptosis. Genes of the 14-3-3 family are conserved from plants to humans, and some members are responsive to UV radiation. Despite the high rate of pollution generated by nano-pollutants, up to now their toxic effect on development is totally obscure. Embryos treated wit...
متن کاملNitric Oxide Mediates the Stress Response Induced by Diatom Aldehydes in the Sea Urchin Paracentrotus lividus
Diatoms are ubiquitous and abundant primary producers that have been traditionally considered as a beneficial food source for grazers and for the transfer of carbon through marine food webs. However, many diatom species produce polyunsaturated aldehydes that disrupt development in the offspring of grazers that feed on these unicellular algae. Here we provide evidence that production of the phys...
متن کاملMetallic nickel nanoparticles and their effect on the embryonic development of the sea urchin Paracentrotus lividus.
The presence of nanoparticles in many industrial applications and daily products is making it nowadays crucial to assess their impact when exposed to the environment. Metallic nickel nanoparticles (Ni NPs) are of high industrial interest due to their ability to catalyze the reversible hydration of CO2 to carbonic acid at ambient conditions. We characterized metallic Ni NPs by XRD, HRTEM and EDS...
متن کاملMolecular Response to Toxic Diatom-Derived Aldehydes in the Sea Urchin Paracentrotus lividus
Diatoms are dominant photosynthetic organisms in the world's oceans and represent a major food source for zooplankton and benthic filter-feeders. However, their beneficial role in sustaining marine food webs has been challenged after the discovery that they produce secondary metabolites, such as polyunsaturated aldehydes (PUAs), which negatively affect the reproductive success of many invertebr...
متن کاملSequence and expression of Paracentrotus lividus alpha tubulin gene.
In an effort to study the regulation of tubulin gene transcription during sea urchin embryogenesis we isolated several genomic and cDNA clones encoding for a and j3 polypeptides (1,2). Here we report the sequence of a full length a tubulin cDNA. Comparison of the predicted amino acid sequence of the cDNA clone (a 1 tubulin), shown in Fig. 1, with that of other clones encoding for a different a ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
international journal of molecular and cellular medicineجلد ۱، شماره ۱، صفحات ۳۰-۳۸
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023